Lucarelli G, Andreani M, Agostinelli F, Manna M, Moretti L, Polchi P
Blut. 1981 Jun;42(6):337-44. doi: 10.1007/BF00996896.
This study investigates the effect of fetal liver transplantation in reconstituting hemopoiesis in supralethally irradiated rats. Different cell doses of fetuses at the embryonic age of 15 and 18 days were compared to equivalent cell doses of adult bone marrow cells. Although the frequency of engraftment ranged between 75 and 100% in all the groups of animals studied, the survival rate at 30 days after TBI did not show any significant difference between the fetal liver and the bone marrow treated recipients. The bone marrow transplants performed in littermate rats almost doubled the percentage of survivors at 30 days and showed a cell dose relationship suggesting that, in the closed colony of random-bred rats used, the mortality after bone marrow and fetal liver transplants was mainly due to graft-versus-host-disease. Antibiotic prophylaxis and treatment during the experiment did not modify the results in a separate group of fetal liver and bone marrow transplanted rats. In the rat model system used in this set of experiments fetal liver did not reveal any advantage over bone marrow transplantation.
本研究调查了胎肝移植对超致死剂量照射大鼠造血重建的影响。将15天和18天胚胎期胎儿的不同细胞剂量与等量成年骨髓细胞剂量进行比较。尽管在所有研究的动物组中植入频率在75%至100%之间，但全身照射后30天的存活率在接受胎肝和骨髓治疗的受体之间未显示出任何显著差异。在同窝大鼠中进行的骨髓移植使30天存活者的百分比几乎增加了一倍，并显示出细胞剂量关系，这表明在所用的随机繁殖大鼠封闭群体中，骨髓和胎肝移植后的死亡率主要归因于移植物抗宿主病。实验期间的抗生素预防和治疗在另一组接受胎肝和骨髓移植的大鼠中未改变结果。在这组实验所用的大鼠模型系统中，胎肝相对于骨髓移植未显示出任何优势。
