二价阳离子增加[3H] - 前列环素与神经元体细胞杂交细胞膜的结合。
Divalent cations increase [3H]-prostacyclin binding to membranes of neuronal somatic hybrid cells.
作者信息
Blair I A, Cresp T M, MacDermot J
出版信息
Br J Pharmacol. 1981 Jul;73(3):691-4. doi: 10.1111/j.1476-5381.1981.tb16804.x.
Abstract
1 [3H]-prostacyclin binding to membranes of a highly differentiated neuronal somatic hybrid is increased equally in the presence of Ca2+, Ba2+, Mg2+ or Sr2+ ions. 2 Analysis of the binding of [3H]-prostacyclin in the presence of low (1 mM) or high (50 mM) Mg2+ ion concentrations has revealed a cation-dependent increase in ligand receptor affinity (Kd values = 57.4 nM and 21.9 nM). 3 The increase in [3H]-prostacyclin binding due to divalent cations is not accompanied by an alteration in the maximum binding capacity of the membranes.
摘要
- [3H] -前列环素与高度分化的神经元体细胞杂种膜的结合，在Ca2 +、Ba2 +、Mg2 +或Sr2 +离子存在时均有同等程度的增加。2. 在低（1 mM）或高（50 mM）Mg2 +离子浓度存在下对[3H] -前列环素结合的分析表明，配体受体亲和力存在阳离子依赖性增加（Kd值分别为57.4 nM和21.9 nM）。3. 二价阳离子导致的[3H] -前列环素结合增加，并未伴随着膜最大结合容量的改变。
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