从猪肾皮质中分离出的肾素抑制剂的特性。

Properties of a renin inhibitor isolated from the pig kidney cortex.

Sagnella G A, Peart W S

Clin Sci (Lond). 1981 Jun;60(6):639-51. doi: 10.1042/cs0600639.

Abstract

A renin-inhibitory material has been partially purified from soluble extracts of the pig kidney cortex by ammonium sulphate precipitation and diethylaminoethylcellulose (DEAE) chromatography and its properties studied. 2. It displayed competitive type kinetics. It did not inhibit cathepsin D, carboxypeptidase A, pancreatic kallikrein or trypsin. 3. Renins from dogs, rabbit and rat were inhibited, but not those from sheep or man, when assayed with pig angiotensinogen. 4. The material was inactivated by treatment with trypsin, N-ethylmaleimide or p-chloromercuribenzoate. 5. Renin-inhibitory activity was not found in plasma from peripheral blood of pigs. 6. It is concluded that the function of the renin inhibitor in the renal cortex of the pig may be restricted to the intrarenal environment.

摘要

一种肾素抑制物质已通过硫酸铵沉淀和二乙氨基乙基纤维素（DEAE）色谱法从猪肾皮质的可溶性提取物中部分纯化，并对其性质进行了研究。2. 它表现出竞争性动力学类型。它不抑制组织蛋白酶D、羧肽酶A、胰激肽释放酶或胰蛋白酶。3. 当用猪血管紧张素原进行测定时，来自狗、兔和大鼠的肾素受到抑制，但来自绵羊或人的肾素未受抑制。4. 该物质经胰蛋白酶、N - 乙基马来酰亚胺或对氯汞苯甲酸处理后失活。5. 在猪外周血的血浆中未发现肾素抑制活性。6. 得出的结论是，猪肾皮质中肾素抑制剂的功能可能仅限于肾内环境。