Branski D, Lebenthal E, Freeman A I, Hatch T F
Digestion. 1981;22(1):8-15. doi: 10.1159/000198589.
A leukemic mouse model was employed to elucidate the separate effect of leukemia and cytotoxic drugs on the jejunal mucosa and its associated digestive enzymes. The mitotic activity, depth of the crypt and villus-crypt quotient were not significantly changed in leukemic mice in comparison to normal mice. The mitotic activity and the depth of the crypt 48 h after 20 mg methotrexate (MTX)/kg were significantly reduced (p less than 0.01) in leukemic mice. Sucrase (p less than 0.001) and maltase (p less than 0.025) activities in the jejunum from leukemic mice were significantly elevated in comparison with non-leukemic controls. In both non-leukemic and leukemic mice, the dose-response curves for MTX administration revealed a significant decrease and a nadir in sucrase (p less than 0.001) and maltase (p less than 0.0025) activities at the dosage of 20 mg/kg. Thus, in the mouse model, leukemia per se does not contribute to significant diminution in small intestinal function. In the small intestine, MTX appears to be responsible for a decrease in the mitotic activity of crypt cells, depth of the crypt and diminished sucrase and maltase activities.
采用白血病小鼠模型来阐明白血病和细胞毒性药物对空肠黏膜及其相关消化酶的单独作用。与正常小鼠相比，白血病小鼠的有丝分裂活性、隐窝深度和绒毛-隐窝比值没有显著变化。白血病小鼠在给予20mg甲氨蝶呤（MTX）/kg后48小时，有丝分裂活性和隐窝深度显著降低（p<0.01）。与非白血病对照组相比，白血病小鼠空肠中的蔗糖酶（p<0.001）和麦芽糖酶（p<0.025）活性显著升高。在非白血病和白血病小鼠中，MTX给药的剂量反应曲线显示，在20mg/kg剂量下，蔗糖酶（p<0.001）和麦芽糖酶（p<0.0025）活性显著下降并出现最低点。因此，在该小鼠模型中，白血病本身并不会导致小肠功能显著降低。在小肠中，MTX似乎是导致隐窝细胞有丝分裂活性降低、隐窝深度减小以及蔗糖酶和麦芽糖酶活性降低的原因。
