Gordenin D A, Inge-Vechtomov S G
Genetika. 1981;17(5):822-31.
Ultraviolet light (UV) at 3000 ergs/mm-2 induces ade2 mutants with a frequency about 10(-4) in wild-type haploid strains of yeast and about 10(-5) in diploid wild-type strains. UV irradiation effectively induced mitotic segregation of ade2 in the heterozygous diploid (the frequency of segregation is 6%). Interallelic complementation and localization spectra are similar for mutations induced both in haploids and diploids. The occurrence of ade2 mutants in diploids correlated with mitotic segregation of the marker his8 which is situated in the same arm of XY chromosome as ade2 is, distal to the centromere. Our data about the frequency of ade2 mutants in diploids and haploids, the frequency of ade2 mitotic segregation, mitotic segregation of other markers and genetic characteristics of ade2 mutations confirm the suggestion that the major mechanism of diploid ade2 mutants appearance is mutation in one of the two ADE2 alleles and consequent mitotic homozygotisation of mutation as a result of mitotic crossingover between ade2 and the centromere.
3000尔格/平方毫米的紫外线（UV）在酵母野生型单倍体菌株中诱导ade2突变体的频率约为10^(-4)，在野生型二倍体菌株中约为10^(-5)。紫外线照射有效地诱导了杂合二倍体中ade2的有丝分裂分离（分离频率为6%）。单倍体和二倍体中诱导的突变的等位基因间互补和定位谱相似。二倍体中ade2突变体的出现与位于XY染色体与ade2相同臂上、着丝粒远端的标记his8的有丝分裂分离相关。我们关于二倍体和单倍体中ade2突变体频率、ade2有丝分裂分离频率、其他标记的有丝分裂分离以及ade2突变的遗传特征的数据证实了以下观点：二倍体ade2突变体出现的主要机制是两个ADE2等位基因之一发生突变，随后由于ade2与着丝粒之间的有丝分裂交换导致突变的有丝分裂纯合化。
