Moroi Y, Hartman A L, Nakane P K, Tan E M
J Cell Biol. 1981 Jul;90(1):254-9. doi: 10.1083/jcb.90.1.254.
Antigens associated with mammalian centromeres were localized at the high and electron microscopic levels using the peroxidase-labeled antibody method. The antibody used was of a type naturally occurring in the sera of patients with scleroderma. At the light microscopic level, it reacts specifically with the centromere regions of chromosomes in a variety of mammalian species and strains in discrete foci in interphase nuclei. We find that the number of foci approximates the number of chromosomes present in the various cell types. At the ultrastructural level, the antigenic foci are confirmed to lie in the kinetochore regions of each chromosome. In interphase nuclei, the antigenic foci were usually associated either with the inner surfaces of the nuclear envelope or with the nucleoli. These observations indicate that the centromere regions of the chromosomes in interphase are not randomly distributed within the nucleus but are usually fixed either to the inner surface of the nuclear envelope or to nucleoli.
利用过氧化物酶标记抗体法，在光学显微镜和电子显微镜水平上对与哺乳动物着丝粒相关的抗原进行了定位。所使用的抗体是硬皮病患者血清中天然存在的一种类型。在光学显微镜水平上，它能与多种哺乳动物物种和品系的染色体着丝粒区域在间期核中的离散位点发生特异性反应。我们发现位点的数量近似于各种细胞类型中存在的染色体数量。在超微结构水平上，抗原位点被证实位于每条染色体的动粒区域。在间期核中，抗原位点通常与核膜的内表面或核仁相关联。这些观察结果表明，间期染色体的着丝粒区域在细胞核内并非随机分布，而是通常固定于核膜的内表面或核仁上。
