一种用于检测二羟苯乙醇酸、二羟苯乙二醇和二羟苯乙酸的灵敏放射酶分析法。

A sensitive radioenzymatic assay for dihydroxymandelic acid, dihydroxypenylglycol and dihydroxyphenylacetic acid .

作者信息

Nagel-Hiemke M, Schümann H J

出版信息

J Biochem Biophys Methods. 1981 Jun;4(5-6):261-70. doi: 10.1016/0165-022x(81)90066-x.

PMID:7019296

Abstract

A sensitive radioenzymatic procedure for the simultaneous determination of dihydroxymandelic acid, dihydroxyphenylglycol, and dihydroxyphenylacetic acid has been developed. The catechols were methylated by catechol-O-methyltransferase using S-adenosyl[methyl-3H]methionine as methyl donor. The assay involved extraction into organic solvent, thin-layer chromatography and periodate oxidation. The applicability of the procedure was tested by measuring the amounts of deaminated metabolites of noradrenaline, adrenaline and dopamine in heart perfusion samples and plasma of rabbits.

摘要

已开发出一种灵敏的放射酶法，用于同时测定二羟基扁桃酸、二羟基苯乙二醇和二羟基苯乙酸。使用S-腺苷-L-[甲基-³H]蛋氨酸作为甲基供体，儿茶酚通过儿茶酚-O-甲基转移酶进行甲基化。该测定包括用有机溶剂萃取、薄层色谱法和高碘酸盐氧化。通过测量兔心脏灌注样品和血浆中去甲肾上腺素、肾上腺素和多巴胺的脱氨基代谢物的量来测试该方法的适用性。