Osinowo O A
J Reprod Fertil. 1981 Jul;62(2):549-54. doi: 10.1530/jrf.0.0620549.
Washing of bull and ram spermatozoa resulted in significant losses of lactic dehydrogenase (LDH) and glucose phosphate isomerase (GPI) from the cell suspensions. Re-suspension of washed bull spermatozoa caused an immediate release of enzymes from the cells. Preincubation of washed ram spermatozoa with 0.025% formaldehyde increased GPI levels but decreased LDH concentration in the extracellular fluid while hexokinase release was unaffected. Varying the incubation temperature between 20 and 37 degrees C affected extracellular LDH and GPI levels. It is suggested that enzyme release from spermatozoa may occur in the absence of any apparent cellular damage.
对公牛和公羊的精子进行洗涤后，细胞悬液中的乳酸脱氢酶（LDH）和葡萄糖磷酸异构酶（GPI）显著损失。洗涤后的公牛精子重新悬浮会导致细胞立即释放酶。用0.025%甲醛对洗涤后的公羊精子进行预孵育，可提高细胞外液中GPI水平，但降低LDH浓度，而己糖激酶的释放不受影响。将孵育温度在20至37摄氏度之间变化会影响细胞外LDH和GPI水平。研究表明，精子释放酶可能在没有任何明显细胞损伤的情况下发生。
