Pauli B U, Memoli V A, Kuettner K E
J Natl Cancer Inst. 1981 Jul;67(1):65-73.
The resistance of cartilage to tumor invasion was studied with the use of a novel in vitro culture system. Articular cartilage obtained from fresh metacarpophalangeal joints of preadolescent bovines was used as a growth surface for human TE-85 osteosarcoma cells and foreskin fibroblasts. Cartilage disks formed the bottoms of stainless-steel cylinders, providing closed growth chambers for these cells. Both invasive osteosarcoma cells and normal fibroblasts were unable to penetrate viable, unextracted cartilage during a 2-week culture period. When cartilage was devitalized by freezing and thawing, the tissue remained resistant to invasion. Cartilage, extracted with either 1 or 3 M guanidine hydrochloride, was invaded by osteosarcoma cells, but not by control fibroblasts. Invasion by osteosarcoma cells into salt-extracted cartilage was abolished when low concentrations of a cartilage-derived, anti-invasion factor were added to the culture medium. These data provided evidence that the resistance of cartilage to tumor invasion is regulated in part by tissue-derived proteinase inhibitors.
利用一种新型体外培养系统研究了软骨对肿瘤侵袭的抵抗作用。取自青春期前牛新鲜掌指关节的关节软骨用作人TE - 85骨肉瘤细胞和包皮成纤维细胞的生长表面。软骨盘构成不锈钢圆柱体的底部，为这些细胞提供封闭的生长室。在为期2周的培养期内，侵袭性骨肉瘤细胞和正常成纤维细胞均无法穿透有活力的、未提取的软骨。当软骨通过冻融失活时，该组织仍具有抗侵袭能力。用1 M或3 M盐酸胍提取的软骨会被骨肉瘤细胞侵袭，但不会被对照成纤维细胞侵袭。当向培养基中添加低浓度的软骨源性抗侵袭因子时，骨肉瘤细胞对盐提取软骨的侵袭就会被消除。这些数据表明，软骨对肿瘤侵袭的抵抗作用部分受组织源性蛋白酶抑制剂的调节。
