Ter-Avanesian M D, Soĭdla T R
Mol Biol (Mosk). 1981 May-Jun;15(3):485-516.
The review considers the results of both genetical and biochemical studies of translation termination in pro- and eukaryotes. The available information on the components of the protein synthesis machinery, participating in the termination process is summarized. Special attention is paid to the problem of nonsense codon recognition. The possibility of modulation of the process of termination in vivo and in vitro is discussed. All the data considered allow us to propose the hypothesis about the role of the small ribosomal subunit RNA (SrRNA) in translation of natural messengers. Deficiency of AGG and related codone in prokaryotes suggests the possibility of scanning of mRNA's in the coding frame by the 3'-terminus of SrRNA. The context of natural terminators in mRNA's in pro-and eukaryotes reveals that the sequences between 6th and 20th positions both up- and down stream from the nonsense codons are complementary to the 3'-end of SrRNA. Interaction between 3'-terminus of SrRNA and the sequences under consideration is postulated to be important for high efficiency termination of translation.
本综述探讨了原核生物和真核生物中翻译终止的遗传学和生物化学研究结果。总结了参与终止过程的蛋白质合成机制各组分的现有信息。特别关注了无义密码子识别问题。讨论了体内和体外调节终止过程的可能性。综合所有这些数据，我们提出了关于小核糖体亚基RNA（SrRNA）在天然信使翻译中作用的假说。原核生物中AGG及相关密码子的缺失表明SrRNA的3'末端有可能在编码框中扫描mRNA。原核生物和真核生物mRNA中天然终止子的上下文显示，无义密码子上下游第6至20位之间的序列与SrRNA的3'末端互补。推测SrRNA的3'末端与所考虑序列之间的相互作用对于高效终止翻译很重要。
