McGuire J C, Morton D R, Brokaw F C, Sun F F
Prostaglandins. 1981 Apr;21(4):609-14. doi: 10.1016/0090-6980(81)90009-5.
(5E)-6a-Carbaprostaglandin I2 (carbacyclin) was oxidized to (5E)-15-dehydro-6a-carbaprostaglandin I2 (15-dehydrocarbacyclin) by partially purified rhesus monkey lung prostaglandin dehydrogenase (PGDH). The (5E-15-dehydro-6a-carbaprostaglandin I2 was isolated by preparative thin-layer chromatography and identified by gas chromatography-mass spectrometry. A Lineweaver-Burke plot gave an apparent Km value of 2.9 microM and a Vmax of 35.7 nmoles carbacyclin oxidized/mg protein/min. These values are similar to previously reported Km and Vmax values for PGI2 and PGE1.
（5E）-6a-卡前列素I2（卡前列环素）被部分纯化的恒河猴肺前列腺素脱氢酶（PGDH）氧化为（5E）-15-脱氢-6a-卡前列素I2（15-脱氢卡前列环素）。通过制备型薄层色谱法分离出（5E）-15-脱氢-6a-卡前列素I2，并通过气相色谱-质谱法进行鉴定。Lineweaver-Burke图给出的表观Km值为2.9微摩尔，Vmax为35.7纳摩尔卡前列环素氧化/毫克蛋白质/分钟。这些值与先前报道的PGI2和PGE1的Km和Vmax值相似。
