Brooks K H, Feldbush T L
J Immunol. 1981 Sep;127(3):959-63.
An in vitro model for the propagation and expansion of the memory B lymphocyte population is described. DNP-BGG immune cells were mixed with OVA immune cells and challenged immediately with DNP-OVA. After the 1st response had begun to wane, the cells were rechallenged with DNP-OVA (day 11 of culture). An average of 13-fold more PFC were observed after delayed challenge (day 11). This expansion in the PFC response was an antigen-dependent process and did not involve recruitment of new memory cells from the virgin lymphocyte pool. The level of expansion of the memory cell pool was also calculated using limiting dilution analysis and was found to fall in a range of 16- to 67-fold increase in precursor frequency. In addition to the expansion of the memory B cell population, we also observed the development of 2 immunoregulatory cycles previously observed only in vivo. First, in the presence of persistent antigen, a cyclical PFC response was seen. Second, after day 10 of culture, optimal PFC numbers were observed only when DNP-lysine was added to the plaque assay. Such hapten-augmentable PFC responses have been reported by other investigators as indicative of anti-idiotypic regulation. This possibility is examined more extensively in the following communication.
本文描述了一种用于记忆B淋巴细胞群体增殖和扩增的体外模型。将二硝基苯-牛γ球蛋白（DNP-BGG）免疫细胞与卵清蛋白（OVA）免疫细胞混合，并立即用二硝基苯-卵清蛋白（DNP-OVA）进行刺激。在初次反应开始减弱后，用DNP-OVA再次刺激细胞（培养第11天）。延迟刺激（第11天）后观察到的分泌抗体细胞（PFC）平均增加了13倍。PFC反应的这种扩增是一个抗原依赖性过程，不涉及从未成熟淋巴细胞池中招募新的记忆细胞。还使用有限稀释分析计算了记忆细胞池的扩增水平，发现前体细胞频率增加了16至67倍。除了记忆B细胞群体的扩增外，我们还观察到了之前仅在体内观察到的两个免疫调节周期的发展。首先，在存在持续抗原的情况下，观察到了周期性的PFC反应。其次，在培养第10天后，仅当在空斑试验中加入二硝基苯赖氨酸（DNP-lysine）时，才观察到最佳的PFC数量。其他研究者报道这种半抗原可增强的PFC反应表明存在抗独特型调节。在以下的交流中对这种可能性进行了更广泛的研究。
