[游离及固定化动物α-L-岩藻糖苷酶的底物特异性及某些性质]

[Substrate specificity and some properties of free and immobilized animal alpha-L-fucosidase].

作者信息

Beĭer E M

出版信息

Biokhimiia. 1977 May;42(5):881-9.

Abstract

The effect of a partially purified preparation of pig kidney alpha-L-fucosidase on some glycoproteins--human and rabbit gamma-globulin, glycoprotein from sheep submaxillary gland and ceruloplasmin--was studied. It was shown that the action of the enzyme of the glycoproteins was not accompanied by a release of fucose. A comparative study of the properties of free and concanavalin A-Sepharose 4B-bound alpha-L-fucosidase was done. The experimental data is indicative of difference in the pH-dependenced and thermostability of these two enzyme forms. It was found that bound alpha-L-fucosidase, similar to the free form, did not split off fucose from the native blood group substances. The data of isoelectric fucosing of alpha-L-fucosidase suggests the existence of enzyme polymorphism.

摘要

研究了猪肾α-L-岩藻糖苷酶部分纯化制剂对一些糖蛋白——人及兔γ-球蛋白、绵羊颌下腺糖蛋白和铜蓝蛋白——的作用。结果表明，该酶对糖蛋白的作用并未伴随岩藻糖的释放。对游离型和伴刀豆球蛋白A-Sepharose 4B结合型α-L-岩藻糖苷酶的性质进行了比较研究。实验数据表明这两种酶形式在pH依赖性和热稳定性方面存在差异。发现结合型α-L-岩藻糖苷酶与游离型相似，不会从天然血型物质中裂解出岩藻糖。α-L-岩藻糖苷酶的等电聚焦数据表明存在酶的多态性。