Pazdernik T L, Uyeki E M
J Pharmacol Exp Ther. 1978 Oct;207(1):255-61.
A new technique is described in which L-1210 colony-forming cells and myelopoietic colony-forming cells can be assessed simultaneously from hemopoietic organs of L-1210-bearing mice. This technique was utilized to evaluate the effects of cyclophosphamide on the recovery of myelopoietic function in tumor-bearing mice. In general, changes in L-1210 colony-forming cells in bone marrows and spleens of cyclophosphamide-treated L-1210-bearing mice correlated with their mean survival time. Injection of L-1210 cells alone enhanced myelopoiesis in the spleen and injection of both cyclophosphamide and L-1210 cells resulted in a synergistic enhancement of myelopoiesis in the spleen. The results in this paper are also compatible with the hypothesis that the host defense system does regulate the growth of L-1210 cells in BDF mice.
本文描述了一种新技术，利用该技术可同时评估携带L-1210小鼠造血器官中的L-1210集落形成细胞和骨髓集落形成细胞。该技术用于评估环磷酰胺对荷瘤小鼠骨髓造血功能恢复的影响。一般来说，环磷酰胺处理的携带L-1210小鼠的骨髓和脾脏中L-1210集落形成细胞的变化与其平均存活时间相关。单独注射L-1210细胞可增强脾脏中的骨髓生成，同时注射环磷酰胺和L-1210细胞可导致脾脏中骨髓生成的协同增强。本文的结果也与宿主防御系统确实调节BDF小鼠中L-1210细胞生长的假说相符。
