Demonstration of particulate accumulations in solid tissue.

We describe a histological clearing procedure involving partial tissue hydrolysis by pancreatin and demonstrate its application. Changes in distribution of 5.7-micrometers latex particles in mouse liver and heart muscle 6 months after i.v. latex injection were quantitated. The method is useful for studied of long-term redistribution of particles in vivo and for locating large, infrequent particles which are difficult to find by ordinary tissue-sectioning technique.