Stenman S, Vaheri A
Int J Cancer. 1981;27(4):427-35. doi: 10.1002/ijc.2910270403.
Fibronectin in human solid tumors was studied by indirect immunofluorescence staining of biopsy material. Altogether 73 tumors were examined, comprising 12 sarcomas, 3 melanomas 1 reticulum cell sarcoma, 39 carcinomas, 6 benign soft-tissue tumors and 12 benign epithelial tumors. In all sarcomas the individual tumor cells were surrounded by a network of fibronectin which was continuous with the stroma. The distribution of fibronectin was similar in the benign soft-tissue tumors. In contrast, no fibronectin was detected in the individual carcinoma cells or in their periphery. However, the reactive connective tissue stroma of carcinomas was strongly connective tissue stroma of carcinomas was strongly positive for fibronectin. This was true also for the stroma of benign epithelial tumors. These results show that, contrary to the situation in cell culture, in vivo sarcoma cells and benign soft-tissue tumor cells contain fibronectin in their pericellular matrix. On the other hand, fibronectin can be used to distinguish carcinomas from sarcomas in vivo.
通过对活检材料进行间接免疫荧光染色，研究了人类实体瘤中的纤连蛋白。共检查了73个肿瘤，包括12个肉瘤、3个黑色素瘤、1个网状细胞肉瘤、39个癌、6个良性软组织肿瘤和12个良性上皮肿瘤。在所有肉瘤中，单个肿瘤细胞被与基质连续的纤连蛋白网络所包围。良性软组织肿瘤中纤连蛋白的分布相似。相比之下，在单个癌细胞或其周边未检测到纤连蛋白。然而，癌的反应性结缔组织基质对纤连蛋白呈强阳性。良性上皮肿瘤的基质也是如此。这些结果表明，与细胞培养的情况相反，在体内肉瘤细胞和良性软组织肿瘤细胞在其细胞周围基质中含有纤连蛋白。另一方面，纤连蛋白可用于在体内区分癌和肉瘤。
