Nishino T, Hata S, Taketani S, Yabusaki Y, Katsuki H
J Biochem. 1981 May;89(5):1391-6. doi: 10.1093/oxfordjournals.jbchem.a133330.
The subcellular distribution of enzymes involved in the reaction sequence from zymosterol to ergosterol was studied. The spheroplasts obtained from cells aerobically grown on ethanol were gently disrupted and the homogenate was fractionated into the subcellular organelles by differential centrifugation. Inspection of the distribution of several marker enzymes revealed that the fractionation was reasonably effected. As a result of experiments, delta 8-delta 7-sterol isomerase, S-adenosylmethionine: delta 24-sterol methyltransferase and the enzyme involved in the reaction sequence from episterol to ergosterol were localized in microsomes. These results suggest the localization of enzymes involved in the late stage of ergosterol synthesis in microsomes.
研究了从酵母甾醇到麦角甾醇反应序列中相关酶的亚细胞分布。从在乙醇上需氧生长的细胞中获得的原生质球被轻轻破碎，匀浆通过差速离心法分离成亚细胞细胞器。对几种标记酶分布的检查表明，分级分离效果良好。实验结果表明，δ8-δ7-甾醇异构酶、S-腺苷甲硫氨酸：δ24-甾醇甲基转移酶以及从表甾醇到麦角甾醇反应序列中涉及的酶定位于微粒体中。这些结果表明麦角甾醇合成后期相关酶定位于微粒体中。
