Ennever J, Streckfuss J L, Goldschmidt M C
J Dent Res. 1981 Oct;60(10):1793-6. doi: 10.1177/00220345810600101001.
Seven ATCC strains of bacteria were examined for apatite formation in a chemically-defined calcification-supporting medium and also in a metastable calcium phosphate solution. One, E. coli, calcified in both. One, S. aureus, calcified in the solution, but not in the medium. The other five did not calcify in either. The results substantiate the belief that calcification is restricted to certain microorganisms. However, they do not rule out the possibility that a noncalcifiable microorganism has the potential to calcify, and the activity is prevented by a cell component. Additionally, the findings emphasize that determining microbiologic calcifiability only in a calcification-supporting culture medium is inadequate. In culture, an efficient calcium pump might preclude calcification by establishing a cytoplasmic calcium level too low for nucleation activation. Calcifiability assays should be done by incubating minimally-metabolizing freeze-dried cells in metastable calcium phosphate solution.
对7株美国典型培养物保藏中心（ATCC）的细菌菌株进行了检测，观察它们在化学组成明确的钙化支持培养基以及亚稳态磷酸钙溶液中形成磷灰石的情况。其中一株大肠杆菌在两种环境中均发生了钙化。一株金黄色葡萄球菌在溶液中发生了钙化，但在培养基中未发生钙化。另外五株在两种环境中均未发生钙化。这些结果证实了钙化仅限于某些微生物的观点。然而，它们并未排除非钙化微生物具有钙化潜力但该活性被细胞成分所抑制的可能性。此外，研究结果强调仅在钙化支持培养基中确定微生物的钙化能力是不够的。在培养过程中，高效的钙泵可能会通过建立过低的细胞质钙水平以激活成核作用，从而阻止钙化。钙化能力检测应通过将最低代谢活性的冻干细胞在亚稳态磷酸钙溶液中孵育来进行。
