Guerraty A, Alivizatos P, Warner M, Hess M, Allen L, Lower R R
J Thorac Cardiovasc Surg. 1981 Oct;82(4):531-7.
A protocol was developed to provide continuous, hypothermic, low-pressure perfusion for 24 hours' preservation of the isolated canine heart prior to orthotopic transplantation. Donor cardiectomy included coronary vasodilatation with diltiazem, potassium arrest, and rapid cooling of the heart. The graft was perfused at a pressure of 18 to 22 cm H2O and at an average flow of 0.743 cc/min/gm of tissue. The septal temperature was 5 degrees to 7 degrees C and perfusate pH was 7.25 to 7.4. Two groups of mongrel dogs were studied after orthotopic transplantation: Group I (n = 15) received hearts perfused for 24 hours. Group II (n = 9) received hearts removed by the same cardiectomy technique, but transplanted immediately. All grafts were able to support the recipient circulation after cardiopulmonary bypass. These was no significant difference in survival or in graft function when hemodynamic studies were done in five animals of each group, between 5 and 10 days after operation. We conclude that a reliable and reproducible method of 24 hours' in vitro perfusion of the canine heart has been obtained and should be applicable in clinical cardiac transplantation when prolonged periods of preservation are required.
制定了一项方案，用于在原位移植前对离体犬心进行24小时的持续低温低压灌注保存。供体心脏切除包括用硫氮䓬酮进行冠状动脉血管扩张、钾停搏以及心脏快速降温。移植物以18至22厘米水柱的压力灌注，组织平均灌注流量为0.743毫升/分钟/克。室间隔温度为5至7摄氏度，灌注液pH值为7.25至7.4。在原位移植后对两组杂种犬进行了研究：第一组（n = 15）接受灌注24小时的心脏。第二组（n = 9）接受通过相同心脏切除技术取出但立即移植的心脏。所有移植物在体外循环后都能够支持受体循环。在每组的5只动物中进行血流动力学研究时，术后5至10天，两组在存活率或移植物功能方面没有显著差异。我们得出结论，已经获得了一种可靠且可重复的犬心24小时体外灌注方法，并且当需要长时间保存时，该方法应适用于临床心脏移植。
