大肠杆菌染色体蛋白的研究。II. 与类核相关的DNA聚合酶。
Studies on Escherichia coli chromosome proteins. II. DNA polymerases associated with the nucleoid.
作者信息
Moriya T, Joh K I, Hori K
出版信息
Biochim Biophys Acta. 1981 Sep 28;655(2):189-94. doi: 10.1016/0005-2787(81)90008-3.
PMID:7025907
Abstract
Escherichia coli nucleoids isolated in the presence of spermidine have been shown to contain DNA polymerase activity of which more than 95% was ascribed to DNA polymerase I and the rest to DNA polymerases II and III. It also has been found that DNA polymerases II and III or DNA polymerase III substituted entirely for DNA polymerase I or DNA polymerase I and II in the nucleoids isolated from E. coli mutants lacking the corresponding enzyme activities.
摘要
已证明在亚精胺存在下分离得到的大肠杆菌类核含有DNA聚合酶活性，其中超过95%归因于DNA聚合酶I，其余归因于DNA聚合酶II和III。还发现，在从缺乏相应酶活性的大肠杆菌突变体中分离得到的类核中，DNA聚合酶II和III或完全替代DNA聚合酶I的DNA聚合酶III，或DNA聚合酶I和II。
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