Glynn P J
Cytobios. 1981;30(119):153-66.
A method is described for the rapid and sensitive assay of phagocytosis which utilizes radioactively labelled bacteria. Ingestion is proportional to the concentration of amoebae in the incubation mixture and a double reciprocal plot of the initial rate of ingestion versus bacterial concentration yields a straight line. In the presence of excess bacteria, the kinetics of ingestion are approximately linear during the early stages of the time course, and then continue at a steadily decreasing rate, eventually levelling off after about 60 min incubation. At this stage, the average number of bacteria per amoebae is between 65 and 70. Phagocytosis is sensitive to low temperature and inhibitors of aerobic respiration but is unaffected by substances which block glycolysis. Digestion of the engulfed bacteria begins rapidly and can apparently proceed to completion since very little radioactivity is lost to the medium for a period of 8 h following ingestion.
本文描述了一种利用放射性标记细菌快速灵敏地检测吞噬作用的方法。摄取量与孵育混合物中变形虫的浓度成正比，摄取初始速率与细菌浓度的双倒数作图得到一条直线。在细菌过量的情况下，摄取动力学在时间进程的早期阶段近似呈线性，然后以稳定下降的速率持续进行，孵育约60分钟后最终趋于平稳。在此阶段，每个变形虫的细菌平均数量在65到70之间。吞噬作用对低温和好氧呼吸抑制剂敏感，但不受阻断糖酵解的物质影响。被吞噬细菌的消化迅速开始，显然可以进行到完全消化，因为摄取后8小时内培养基中很少有放射性损失。
