诺特蛇毒素优先抑制大鼠脑突触体组分中新合成的乙酰胆碱的释放。
Notexin preferentially inhibits the release of newly synthesized acetylcholine from rat brain synaptosomal fractions.
作者信息
Gundersen C B, Jenden D J
出版信息
J Neurosci. 1981 Oct;1(10):1113-6. doi: 10.1523/JNEUROSCI.01-10-01113.1981.
An investigation was made of the effects of the snake venom neurotoxin, notexin, on acetylcholine turnover in rat brain P2 fractions using a gas chromatographic mass spectrometric assay for acetylcholine and choline. In contrast to earlier reports, we found a stimulation of the uptake and acetylation of labeled choline by toxin-treated P2 fractions. More significantly, notexin inhibited the release of this newly synthesized transmitter. These effects were found to be dependent on the dose of the toxin and the time of exposure of the P2 fraction to notexin. Longer exposure to notexin or experiments involving resuspension of notexin-treated P2 fractions appeared to result in considerable lysis of the transmitter-containing particles. Thus, notexin may alter acetylcholine compartmentation in the nerve ending and thereby affect acetylcholine synthesis.
采用气相色谱 - 质谱分析法测定乙酰胆碱和胆碱，研究了蛇毒神经毒素——诺太毒素对大鼠脑P2组分中乙酰胆碱周转的影响。与早期报告相反，我们发现毒素处理过的P2组分对标记胆碱的摄取和乙酰化有刺激作用。更显著的是，诺太毒素抑制了这种新合成递质的释放。这些作用被发现取决于毒素的剂量以及P2组分与诺太毒素接触的时间。长时间暴露于诺太毒素或涉及重悬经诺太毒素处理的P2组分的实验似乎导致了含递质颗粒的大量裂解。因此，诺太毒素可能会改变神经末梢中乙酰胆碱的分布，从而影响乙酰胆碱的合成。
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