Kaneko S, Moriwaki C
J Pharmacobiodyn. 1981 Jun;4(6):443-50. doi: 10.1248/bpb1978.4.443.
The influences of bradykinin, kallidin and dipeptidyl carboxypeptidase (kininase II) on the motility of human sperm thoroughly washed with Ficoll were investigated by an objective method with multiple exposure photography technique which recorded stroboscopically the tract of sperm migration. Addition of 0.1--10 ng/ml of bradykinin or kallidin to sperm suspension caused a stimulation of motility. The effect appeared by increase of the velocity of forward motile sperm, whereas the percent of motile sperm was not altered. On the contrary, higher dose of kinins suppressed the motility by decreasing the percent of motile sperm. There was a minor contamination of dipeptidyl carboxypeptidase even in the highly washed sperm suspension. This enzyme was identical with angiotensin I converting enzyme or kininase II and the major enzyme of kinin degrading activity in seminal plasma. Stimulation of sperm motility by kinin was further enhanced in the presence of SQ 14225, a specific inhibitor of dipeptidyl carboxypeptidase, by protection of added kinins from the residual dipeptidyl carboxypeptidase in the sperm suspension. Dipeptidyl carboxypeptidase level in ejaculated human semen was found to correlate to the semen quality, such as sperm density and motility, but it gave no direct influence on sperm motility.
采用多次曝光摄影技术以客观方法研究了缓激肽、胰激肽和二肽基羧肽酶（激肽酶II）对用菲可（Ficoll）充分洗涤过的人类精子活力的影响，该技术通过频闪观测记录精子迁移轨迹。向精子悬液中添加0.1 - 10 ng/ml的缓激肽或胰激肽会刺激精子活力。这种作用表现为向前运动精子的速度增加，而活动精子的百分比未改变。相反，较高剂量的激肽通过降低活动精子的百分比来抑制精子活力。即使在高度洗涤过的精子悬液中也存在少量二肽基羧肽酶污染。这种酶与血管紧张素I转换酶或激肽酶II相同，是精浆中激肽降解活性的主要酶。在存在二肽基羧肽酶特异性抑制剂SQ 14225的情况下，通过保护添加的激肽免受精子悬液中残留二肽基羧肽酶的作用，激肽对精子活力的刺激作用进一步增强。发现射出的人类精液中二肽基羧肽酶水平与精液质量（如精子密度和活力）相关，但它对精子活力没有直接影响。
