Atia M, Farid A, Zaki M M
Sabouraudia. 1981 Sep;19(3):217-21. doi: 10.1080/00362178185380341.
140 soil samples from the Assiut governorate were examined for pathogenic fungi and actinomycetes. The paraffin-bait technique and a modified sodium citrate-utilizing procedure were used for recovery of Nocardia strains. Five isolations of N. brasiliensis were made by the sodium citrate method while 3 isolates were obtained by the other method. N. gypsoides was isolated for the first time, only the the modified method. It appears that the latter method suppresses to a great extent the growth of saprophytic fungi, hence increasing the possibility of the isolation of Nocardia spp. The effect of keratin enrichment on the isolation of dermatophytes from soil by the hair-bait technique was also investigated. Pretreatment of samples with dog hair or feathers increased the yield of isolates of Microsporum gypseum, Trichophyton terrestre and to a lesser extent, T. mentagrophytes, M. canis was recovered from soil only after its enrichment with dog hair. It is suggested that enrichment with other forms of keratinous material, either separately or in combination, might increase the recovery of keratinophilic fungi from soil.
对来自阿斯尤特省的140份土壤样本进行了致病真菌和放线菌检测。采用石蜡诱饵技术和改良的柠檬酸钠利用程序来分离诺卡氏菌菌株。通过柠檬酸钠法分离出5株巴西诺卡氏菌，通过另一种方法获得了3株分离株。仅用改良方法首次分离出石膏样诺卡氏菌。看来后一种方法在很大程度上抑制了腐生真菌的生长，从而增加了分离诺卡氏菌属的可能性。还研究了角蛋白富集对毛发诱饵技术从土壤中分离皮肤癣菌的影响。用狗毛或羽毛对样本进行预处理，增加了石膏样小孢子菌、 terrestre毛癣菌的分离产量，对须癣毛癣菌的增加程度较小，犬小孢子菌仅在土壤用狗毛富集后才从土壤中分离出来。建议单独或联合使用其他形式的角蛋白物质进行富集，可能会增加从土壤中分离嗜角质真菌的回收率。
