Huchon G J, Little J W, Murray J F
J Appl Physiol Respir Environ Exerc Physiol. 1981 Oct;51(4):955-62. doi: 10.1152/jappl.1981.51.4.955.
We developed a method for measuring an index of alveolar-capillary membrane permeability (PI) by aerosolizing a mixture of 99mTc-diethylenetriaminepentaacetic acid (Tc-DTPA) and 125I-antipyrine (I-AP) and injecting 111In-DTPA (In-DTPA). The I-AP was used to compute the quantity of Tc-DTPA delivered and the In-DTPA the quantity of Tc-DTPA in the body. The PI was the ratio of the uptake of Tc-DTPA per minute to the amount deposited at the end of aerosolization. In 14 anesthetized dogs we measured the volume of distribution of I-AP (0.54 +/- 0.034 l/kg body wt) and/or showed that the volumes of distribution of Tc-DTPA and In-DTPA were similar. We measured PI in four groups of dogs: control (n = 5), oleic acid (n = 5), hydrochloric acid (n = 6), and high left atrial pressure (n = 5). The PI increased significantly in both groups with acid-induced increased permeability compared with the control and high left atrial pressure groups, which did not differ from each other. We conclude that the aerosolization method is suitable for differentiating increased from normal permeability.
我们研发了一种测量肺泡-毛细血管膜通透性指数（PI）的方法，即雾化99mTc-二乙烯三胺五乙酸（Tc-DTPA）和125I-安替比林（I-AP）的混合物，并注射111In-DTPA（In-DTPA）。I-AP用于计算递送的Tc-DTPA的量，而In-DTPA用于计算体内Tc-DTPA的量。PI是每分钟Tc-DTPA的摄取量与雾化结束时沉积量的比值。在14只麻醉犬中，我们测量了I-AP的分布容积（0.54±0.034 l/kg体重）和/或表明Tc-DTPA和In-DTPA的分布容积相似。我们在四组犬中测量了PI：对照组（n = 5）、油酸组（n = 5）、盐酸组（n = 6）和高左心房压组（n = 5）。与对照组和高左心房压组相比，两组因酸诱导通透性增加导致PI显著升高，而对照组和高左心房压组之间无差异。我们得出结论，雾化法适用于区分通透性增加与正常情况。
