James K, Hansen B, Gewurz H
J Immunol. 1981 Dec;127(6):2545-50.
In the preceding paper we reported that C-reactive protein (CRP) in the presence of a multimeric binding specificity such as C-polysaccharide (CPS) binds to a small percentage of peripheral blood lymphocytes (PBL). In the present paper additional methods for demonstrating this binding were developed and utilized to help characterize the CRP-binding cell. Heat-modified CRP and E-CPS-CRP each were found to bind to a similar percentage of PBL by microscopic visualization, and an identical degree of binding was observed by cytofluorimetric analysis. Multiple marker studies indicated that CRP-binding cells are found in the T cell, B cell, and null cell categories in a ratio of 2:1:1, respectively. Preferential overlap was seen with IgG FcR-bearing cells, which accounted for 70% of the CRP-binding cells; however, only 12% of the FcR cells bound CRP. These studies indicate that CRP-binding cells predominantly represent a subset of cells bearing FcR, and these cells have the morphologic characteristics of large granular lymphocytes. CRP in the presence of CPS bound to cells of multiple human and murine cultured lines shown to have IgG FcR reactivity, but showed lesser or no binding to cell lines negative for FcR. The precise morphologic and functional characterization of the CRP-binding cells and the nature of the binding site are yet to be established.
在之前的论文中我们报道，在存在诸如C-多糖（CPS）这种多聚体结合特异性的情况下，C反应蛋白（CRP）会与一小部分外周血淋巴细胞（PBL）结合。在本文中，我们开发并利用了其他方法来证明这种结合，以帮助对CRP结合细胞进行特征描述。通过显微镜观察发现，热修饰的CRP和E-CPS-CRP各自与相似比例的PBL结合，并且通过细胞荧光分析观察到相同程度的结合。多标记研究表明，CRP结合细胞分别以2:1:1的比例存在于T细胞、B细胞和裸细胞类别中。在带有IgG Fc受体的细胞中观察到优先重叠，这些细胞占CRP结合细胞的70%；然而，只有12%的FcR细胞结合CRP。这些研究表明，CRP结合细胞主要代表带有FcR的细胞亚群，并且这些细胞具有大颗粒淋巴细胞的形态学特征。在存在CPS的情况下，CRP与多种已显示具有IgG FcR反应性的人和小鼠培养细胞系的细胞结合，但与FcR阴性的细胞系结合较少或不结合。CRP结合细胞的确切形态学和功能特征以及结合位点的性质尚未确定。
