来自大肠杆菌的CTP合成酶的底物特异性。
Substrate specificity of CTP-synthetase from E. coli.
作者信息
Scheit K H, Linke H J
出版信息
Nucleic Acids Symp Ser. 1981(9):229-33.
PMID:7029474
Abstract
The substrate specificity of CTP-synthetase from E. coli was investigated by means of UTP analogs. This study revealed that the three main structural elements of the UTP molecule were important for the substrate specificity of the enzyme. CTP-synthetase seems to possess an absolute requirement for the beta-D-ribose 5-triphosphate part in UTP. Substitutents in 5-position of UTP, exceeding the size of a tritium atom abolish substrate function.
摘要
利用尿苷三磷酸（UTP）类似物对大肠杆菌CTP合成酶的底物特异性进行了研究。该研究表明，UTP分子的三个主要结构元件对该酶的底物特异性很重要。CTP合成酶似乎对UTP中的β-D-核糖5-三磷酸部分有绝对需求。UTP 5位上的取代基若超过氚原子的大小，就会消除底物功能。
相似文献
1
Substrate specificity of CTP-synthetase from E. coli.来自大肠杆菌的CTP合成酶的底物特异性。
Nucleic Acids Symp Ser. 1981(9):229-33.
2
Substrate specificity of CTP synthetase from Escherichia coli.大肠杆菌CTP合成酶的底物特异性
Eur J Biochem. 1982 Aug;126(1):57-60. doi: 10.1111/j.1432-1033.1982.tb06745.x.
3
Functional expression of the gene encoding cytidine triphosphate synthetase from Plasmodium falciparum which contains two novel sequences that are potential antimalarial targets.恶性疟原虫胞苷三磷酸合成酶编码基因的功能表达，该基因包含两个潜在抗疟靶点的新序列。
Mol Biochem Parasitol. 2005 Oct;143(2):200-8. doi: 10.1016/j.molbiopara.2005.06.004.
4
Mutational analysis of conserved glycine residues 142, 143 and 146 reveals Gly(142) is critical for tetramerization of CTP synthase from Escherichia coli.对保守甘氨酸残基142、143和146的突变分析表明，甘氨酸（142）对大肠杆菌CTP合酶的四聚化至关重要。
Biochem J. 2008 May 15;412(1):113-21. doi: 10.1042/BJ20071163.
5
Ground state, intermediate, and multivalent nucleotide analogue inhibitors of cytidine 5'-triphosphate synthase.
ChemMedChem. 2008 Dec;3(12):1853-7. doi: 10.1002/cmdc.200800236.
6
Crystal structures of CTP synthetase reveal ATP, UTP, and glutamine binding sites.CTP合成酶的晶体结构揭示了ATP、UTP和谷氨酰胺结合位点。
Structure. 2004 Aug;12(8):1413-23. doi: 10.1016/j.str.2004.05.013.
7
Substrate inhibition of Lactococcus lactis cytidine 5'-triphosphate synthase by ammonium chloride is enhanced by salt-dependent tetramer dissociation.氯化铵对乳酸乳球菌胞苷5'-三磷酸合成酶的底物抑制作用因盐依赖性四聚体解离而增强。
Arch Biochem Biophys. 2003 May 1;413(1):17-22. doi: 10.1016/s0003-9861(03)00085-7.
8
The roles of uridine-cytidine kinase and CTP synthetase in the synthesis of CTP in malignant human T-lymphocytic cells.
Adv Exp Med Biol. 1994;370:261-4. doi: 10.1007/978-1-4615-2584-4_55.
9
Identification of Ser424 as the protein kinase A phosphorylation site in CTP synthetase from Saccharomyces cerevisiae.鉴定Ser424为酿酒酵母CTP合成酶中蛋白激酶A的磷酸化位点。
Biochemistry. 1999 Jul 6;38(27):8839-48. doi: 10.1021/bi990784x.
Zotero 插件