Lautala P, Martin J M
Acta Endocrinol (Copenh). 1981 Dec;98(4):481-7. doi: 10.1530/acta.0.0980481.
In vitro glucose oxidation and glucose transport in the rat medial (MH) and lateral (LH) hypothalamic areas was measured. Glucose oxidation was calculated from the conversion of [U-14C]glucose to 14CO2 and glucose transport from 14CO2 produced from [1-14C]glucose in the presence of phenazine and methosulphate and NaF. Increasing glucose in the medium from 1 mM to 20 mM enhanced glucose oxidation two-fold in MH and 40% in LH. Addition of insulin, 100 microU/ml, to the medium decreased glucose oxidation 30% both in MH and LH at both 4 mM and 20 mM glucose. Fasting did not affect glucose oxidation in either of these hypothalamic areas. Glucose transport was not affected by insulin, but was increased significantly when glucose was raised from 0.25 mM to 1.0 mM. Fasting also increased glucose transport in both hypothalamic areas. In conclusion, extracellular glucose concentration seems to be the major regulator of glucose utilization by the rat hypothalamus. Insulin, rather than increasing, seems to decrease glucose oxidation while having no effect on glucose transport.
测定了大鼠内侧下丘脑（MH）和外侧下丘脑（LH）区域的体外葡萄糖氧化和葡萄糖转运。葡萄糖氧化通过[U-14C]葡萄糖转化为14CO2来计算，葡萄糖转运通过在吩嗪、甲基硫酸酯和氟化钠存在下由[1-14C]葡萄糖产生的14CO2来计算。将培养基中的葡萄糖浓度从1 mM增加到20 mM，可使MH中的葡萄糖氧化增加两倍，使LH中的葡萄糖氧化增加40%。向培养基中添加100微单位/毫升的胰岛素，在葡萄糖浓度为4 mM和20 mM时，可使MH和LH中的葡萄糖氧化均降低30%。禁食对这两个下丘脑区域的葡萄糖氧化均无影响。葡萄糖转运不受胰岛素影响，但当葡萄糖浓度从0.25 mM提高到1.0 mM时，葡萄糖转运显著增加。禁食也增加了两个下丘脑区域的葡萄糖转运。总之，细胞外葡萄糖浓度似乎是大鼠下丘脑葡萄糖利用的主要调节因子。胰岛素似乎不是增加而是降低葡萄糖氧化，同时对葡萄糖转运没有影响。
