Emeruwa A C
Ann Microbiol (Paris). 1981 Jul-Aug;132B(1):13-21.
Sudanophilic and iodine-staining cytoplasmic granules were isolated at various developmental stages during growth of Nocardia asteroides (strain 55) and identified as poly-beta-hydroxybutyrate (PHB) and glycogen granules, respectively. During growth in nutrient broth containing 1% glucose, maximal accumulation of PHB and glycogen granules, up to 10 and 20% respectively, of its dry-weight was obtained in the filamentous cells at 16 h just prior to the onset of cell fragmentation during the stationary phase. The decrease of the cytoplasmic granules was concomitant with fragmentation of the cells to rod-like and spherical cells, suggesting that the polymers may serve as carbon and energy source during morphogenesis. Both granules were detected in the three cell-forms. At higher concentrations of glucose (4%) more glycogen granules (9 times) accumulated than PHB (4 times) and glycogen hydrolysis was also faster than that of PHB, suggesting preferences of glycogen over PHB as energy and carbon source under this growth condition. Growth and biosynthesis of granules were significantly reduced by very high glucose concentration (10%) and the usual pleomorphic developmental stages were reduced to a dimorphic life cycle. Thus, biosynthesis of both granules and morphogenesis are under catabolite repression. Both polymers were also found in N. brasiliensis and N. otitidis-caviarum, indicating that cytoplasmic accumulation of multiple granules is common in the genus.
在星形诺卡氏菌（菌株55）生长的不同发育阶段，分离出嗜苏丹性和碘染色的细胞质颗粒，分别鉴定为聚-β-羟基丁酸酯（PHB）和糖原颗粒。在含有1%葡萄糖的营养肉汤中生长时，在稳定期细胞破碎开始前16小时的丝状细胞中，PHB和糖原颗粒的最大积累量分别达到其干重的10%和20%。细胞质颗粒的减少与细胞破碎成杆状和球状细胞同时发生，这表明这些聚合物可能在形态发生过程中作为碳源和能源。在三种细胞形态中都检测到了这两种颗粒。在较高浓度的葡萄糖（4%）下，积累的糖原颗粒（9倍）比PHB（4倍）多，并且糖原水解也比PHB快，这表明在这种生长条件下，糖原比PHB更受青睐作为能量和碳源。非常高的葡萄糖浓度（10%）显著降低了颗粒的生长和生物合成，并且通常的多形发育阶段减少为双形生命周期。因此，颗粒的生物合成和形态发生都受到分解代谢物阻遏。在巴西诺卡氏菌和耳腔诺卡氏菌中也发现了这两种聚合物，这表明在该属中细胞质中积累多种颗粒是常见的。
