Hughes K T, Coles G A, Harry T R, Davies M
Biochim Biophys Acta. 1981 Nov 13;662(1):111-8. doi: 10.1016/0005-2744(81)90230-8.
The proteinase content of highly purified preparations of human peripheral blood monocytes was investigated. Monocyte cell lysates exhibited activity at neutral pH against azocasein, 3H-labelled elastin as well as several synthetic substrates used to detect serine proteinases (EC 3.4.21.-) of human polymorphonuclear leucocytes. The cell lysates also contain at least two acid proteinases. The levels of neutral proteinase activity in monocytes was considerably less than that found in polymorphonuclear leucocytes. The effect of inhibitors on the monocytes neutral proteinases showed them to be of the serine type. Monocytes also solubilized and degraded the type IV collagen found in human glomerular basement membrane at neutral and acid pH. The action of the monocyte proteinase on glomerular basement membrane indicated that their properties were similar but not identical to that of the polymorphonuclear leucocyte serine proteinases. Since monocytes infiltrate the glomerulus in certain forms of immunologically mediated glomerulonephritis, it may well be that monocyte serine proteinases make a contribution to the glomerular damage that occurs.
对人外周血单核细胞高度纯化制剂中的蛋白酶含量进行了研究。单核细胞裂解物在中性pH值下对偶氮酪蛋白、3H标记的弹性蛋白以及几种用于检测人多形核白细胞丝氨酸蛋白酶（EC 3.4.21.-）的合成底物表现出活性。细胞裂解物还含有至少两种酸性蛋白酶。单核细胞中中性蛋白酶活性水平明显低于多形核白细胞中的水平。抑制剂对单核细胞中性蛋白酶的作用表明它们属于丝氨酸类型。单核细胞在中性和酸性pH值下还能溶解并降解人肾小球基底膜中的IV型胶原。单核细胞蛋白酶对肾小球基底膜的作用表明，它们的特性与多形核白细胞丝氨酸蛋白酶相似但不完全相同。由于单核细胞在某些形式免疫介导的肾小球肾炎中浸润肾小球，很可能单核细胞丝氨酸蛋白酶对所发生的肾小球损伤有一定作用。
