Balmaceda J P, Schally A V, Coy D, Asch R H
Contraception. 1981 Sep;24(3):275-81. doi: 10.1016/0010-7824(81)90040-8.
Seven regularly cycling rhesus monkeys (Macaca mulatta) were used in this experiment. The animals were followed during two consecutive cycles. During the first cycle (control), the animals did not receive any treatment and the date of ovulation was determined by using daily total serum estrogens and serial laparoscopies. In the second cycle, the animals received 1 mg daily of [N-Ac-D-Trp(1)(3),D-p-Cl-Phe(2), D-Phe(6), D-Ala(10)-LH-RH by intramuscular injection from days 10-14. The date of ovulation was determined by using the same methodology as the control cycle. Blood samples were drawn daily from day 8 of the cycle until the onset of menses, and the serum was used to measure total estrogens, progesterone and LH. A significant delay of the preovulatory LH peak and ovulation occurred in 5 of the 7 animals resulting in a proportional increase in cycle length as compared to the control cycle. No changes in cycle length or date of LH peak occurred in the other 2 animals. One of them did not present signs of ovulation as determined by laparoscopy (no recognizable stigma or corpus luteum). Progesterone production and length of the luteal phase were not affected by the treatment.
本实验使用了七只规律排卵的恒河猴（猕猴）。在两个连续周期内对这些动物进行跟踪观察。在第一个周期（对照期），动物未接受任何治疗，通过每日测定血清总雌激素水平和连续腹腔镜检查来确定排卵日期。在第二个周期，从第10天至第14天，动物每天肌肉注射1毫克 [N - 乙酰 - D - 色氨酸(1)(3)，D - 对氯苯丙氨酸(2)，D - 苯丙氨酸(6)，D - 丙氨酸(10)] - 促黄体生成素释放激素（LH - RH）。通过与对照周期相同的方法来确定排卵日期。从周期第8天开始每天采集血样直至月经来潮，血清用于测定总雌激素、孕酮和促黄体生成素（LH）。7只动物中有5只出现排卵前LH峰和排卵明显延迟，与对照周期相比，周期长度成比例增加。另外2只动物的周期长度或LH峰日期没有变化。其中一只经腹腔镜检查未出现排卵迹象（无可识别的排卵点或黄体）。孕酮生成和黄体期长度不受该治疗影响。
