Masters C J
CRC Crit Rev Biochem. 1981;11(2):105-43. doi: 10.3109/10409238109108700.
Soluble enzymes contribute significantly to the metabolic capabilities of living organisms, but it is becoming increasingly clear that the activities of these enzymes are significantly modified by their interactions with structural components of the cells, and that these interactions may make important contributions to metabolic regulation. In the past, specification of these interactions has been limited by the availability of suitable experimental techniques, but this deficiency is now being rectified and our understanding of these processes if advancing rapidly. Research in this area is moving into a second phase, with the emphasis no longer being focused on demonstrations of the biological reality of these interactions, but directed more towards quantitative aspects of binding, the determination of the characteristics of binding domains, and the theoretical basis of regulatory involvements. All of these aspects are discussed in the present review.
可溶性酶对生物体的代谢能力有显著贡献，但越来越明显的是，这些酶的活性会因其与细胞结构成分的相互作用而发生显著改变，并且这些相互作用可能对代谢调节有重要贡献。过去，由于缺乏合适的实验技术，对这些相互作用的描述受到限制，但现在这一缺陷正在得到纠正，我们对这些过程的理解正在迅速推进。该领域的研究正在进入第二阶段，重点不再是证明这些相互作用的生物学真实性，而是更多地转向结合的定量方面、结合结构域特征的确定以及调节参与的理论基础。本综述将讨论所有这些方面。
