泛醌提取对牛心线粒体中泛醇-1氧化酶活性的影响。
Effect of ubiquinone extraction on ubiquinol-1 oxidase activity in beef heart mitochondria.
作者信息
Pasquali P, Landi L, Cabrini L, Lenaz G
出版信息
J Bioenerg Biomembr. 1981 Aug;13(3-4):141-8. doi: 10.1007/BF00763836.
PMID:7031049
Abstract
Extraction of endogenous ubiquinone with different methods does not influence ubiquinol oxidase activity in lyophilized mitochondria in terms of KM, although a decrease of Vmax is sometimes observed. Experiments with submitochondrial particles from a UQ-deficient mutant of S. cerevisiae confirm the results with UQ-depleted mitochondria and support the idea that endogenous ubiquinone is not required for the oxidation of exogenous ubiquinols by complex III.
摘要
采用不同方法提取内源性泛醌，就米氏常数（KM）而言，对冻干线粒体中的泛醇氧化酶活性并无影响，不过有时会观察到最大反应速度（Vmax）降低。对酿酒酵母泛醌缺陷型突变体的亚线粒体颗粒进行的实验，证实了对泛醌耗竭线粒体的实验结果，并支持以下观点：复合物III氧化外源性泛醇并不需要内源性泛醌。
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