2,3-二磷酸甘油酸对凝血酶的体外抑制作用。
The in vitro inhibitory effect on thrombin by 2,3-diphosphoglycerate.
作者信息
Del Principe D, Strappini P M, Del Vecchio S, Menichelli A, Biancini G, Cosmi E V, Bastianon V, D'Arcangelo C
出版信息
Thromb Haemost. 1981 Oct;46(3):581-3.
Thrombin incubated with 2,3-diphosphoglycerate (150 nmol 2,3-DPG/1 NIH thrombin unit) lost up to 70% of its clotting activity, whereas the esterase activity remained unchanged. No fibrinopeptide release by thrombin was observed in the presence of 2,3-DPG. The fibrin polymerization was normal. By chromatography on Amberlite IRC-50, alpha-thrombin was eluted at pH 8.0. In presence of 2,3-DPG, alpha-thrombin was not eluted. Likely, 2,3-DPG can interfere with thrombin.
凝血酶与2,3 - 二磷酸甘油酸（150 nmol 2,3 - DPG/1 NIH凝血酶单位）一起孵育时，其凝血活性丧失高达70%，而酯酶活性保持不变。在2,3 - DPG存在的情况下，未观察到凝血酶释放纤维蛋白肽。纤维蛋白聚合正常。通过在Amberlite IRC - 50上进行色谱分析，α-凝血酶在pH 8.0时被洗脱。在2,3 - DPG存在的情况下，α-凝血酶未被洗脱。很可能，2,3 - DPG会干扰凝血酶。
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