Kol'tsova S V, Shataeva L K, Sukhareva T F, Byniaeva N A, Fedorova Z D
Vopr Med Khim. 1981 Sep-Oct;27(5):623-6.
An improved procedure is developed for isolation of urokinase from human urine by means of ion exchange on cyrboxyl cationite SMT at pH 4.7. with subsequent stepwice elution by phosphate buffer, rH7.0. Depigmentation of the urine was achieved by pretreatment with an anionite FAF. Chromatographically pure preparation of urokinase was obtained as shown by gel filtration through Sephadex G-100 and G-150.
开发了一种改进的方法，用于在pH 4.7条件下通过羧基阳离子交换剂SMT从人尿中分离尿激酶，随后用pH 7.0的磷酸盐缓冲液进行分步洗脱。通过用阴离子交换剂FAF进行预处理实现尿液的脱色。通过Sephadex G - 100和G - 150凝胶过滤显示，获得了色谱纯的尿激酶制剂。
