Feige U
Zentralbl Bakteriol Mikrobiol Hyg A Med Mikrobiol Infekt Parasitol. 1981;250(1-2):52-62.
Lipopolysaccharide from E, coli C as well as lipopolysaccharides from submutants of E. coli with incomplete core structures in their lipopolysaccharides were isolated and quantitatively analyzed. Core oligosaccharides were isolated from lipopolysaccharides by acetic acid degradation and were purified by gel chromatography. The difference in molecular rotations of the core oligosaccharides from E. coli C and 6 submutants thereof with incomplete core structure were correlated to the differences in sugar compositions. The anomeric configurations have been deducted from the high or low contribution of each individual sugar to the molecular rotation of the core oligosaccharide from E. coli C. The primary structure of the hexose region of the lipopolysaccharide from E. coli C is primary structure of the hexose region of the lipopolysaccharide from E. coli C is, see formula in text. The anomeric configurations of glucoses I, II, and III were confirmed by precipitation reactions of alkali treated lipopolysaccharides from E. coli C, C23. 1, and C21 with Concanavalin A. The alpha-anomeric configurations of both the galactoses were confirmed by degradation studies with alpha-galactosidase (E.C.3.2.1.22) from green coffee beans with the isolated and purified core oligosaccharide from E. coli C71.
分离并定量分析了来自大肠杆菌C的脂多糖以及脂多糖核心结构不完整的大肠杆菌亚突变体的脂多糖。通过乙酸降解从脂多糖中分离出核心寡糖，并通过凝胶色谱法进行纯化。将大肠杆菌C及其6个核心结构不完整的亚突变体的核心寡糖的分子旋光度差异与糖组成差异相关联。从每种单糖对大肠杆菌C核心寡糖分子旋光度的高或低贡献中推断出异头构型。大肠杆菌C脂多糖己糖区域的一级结构，见文中公式。通过大肠杆菌C、C23.1和C21的碱处理脂多糖与伴刀豆球蛋白A的沉淀反应，证实了葡萄糖I、II和III的异头构型。通过用来自生咖啡豆的α-半乳糖苷酶（E.C.3.2.1.22）对分离和纯化的大肠杆菌C71核心寡糖进行降解研究，证实了两种半乳糖的α-异头构型。
