Schott F J, Grubert M, Wangler W, Ackermann T
Biophys Chem. 1981 Sep;14(1):25-30. doi: 10.1016/0301-4622(81)87003-2.
The heat effects involved in thermal unfolding of five tRNAs with different primary structures have been determined by direct differential scanning microcalorimetry. The overall molar values of the transition enthalpy (delta Ht) are 1150 kJ/mol for tRNA Lys2 (yeast), 1250 kJ/mol for tRNA Phe (yeast), 1350 kJ/mol for tRNA Val (yeast), 1490 kJ/mol for tRNA Val (E. coli) and 1630 kJ/mol for tRNA Tyr (E. coli). The tRNAs differ in their melting behaviour as can be shown by a comparison of the calorimetric curves. The calorimetrically measured delta Ht values are about 350 kJ/mol higher than the transition enthalpy values for the cloverleaf arrangement, which were estimated using the known parameters for G.C and A.U base pairs.
通过直接差示扫描量热法测定了五种具有不同一级结构的tRNA热解折叠过程中的热效应。对于酵母的赖氨酸tRNA（tRNA Lys2），转变焓（ΔHt）的总摩尔值为1150 kJ/mol；酵母的苯丙氨酸tRNA（tRNA Phe）为1250 kJ/mol；酵母的缬氨酸tRNA（tRNA Val）为1350 kJ/mol；大肠杆菌的缬氨酸tRNA（tRNA Val）为1490 kJ/mol；大肠杆菌的酪氨酸tRNA（tRNA Tyr）为1630 kJ/mol。通过比较量热曲线可以看出，这些tRNA的解链行为有所不同。量热法测得的ΔHt值比使用已知的G.C和A.U碱基对参数估算的三叶草结构的转变焓值高约350 kJ/mol。
