Komissarenko V P, Turchin I S, Lazareva N P
Biull Eksp Biol Med. 1981 Oct;91(10):489-91.
Monolayer cultures of pancreatic islets obtained from the pancreas of guinea-pigs aged 10 days and 3-3.5-months pig embryos were studied at the 2d-3d, 5th-6th and 15th-16th days. These times corresponded with the stages of culture intense growth, stabilization and physiological dying off. The rate of insulin biosynthesis and secretory activity of beta-cells were evaluated from the intensity of 3H-uridine and 3H-leucine incorporation and from the magnitude of immunoreactive insulin secretion into the cultural medium. The proliferating and insulin-synthetizing beta-cells were detected by selective 3H-thymidine or 3H-leucine incorporation. In the stages of culture intense growth and dying off the viable beta-cells were distributed into insulin-synthesizing and proliferating ones. During the transition to the stabilization stage the proliferative level of beta-cells drastically decreased, while the biosynthesis and insulin secretion rates increased. This manifested in the increased number of beta-cells which intensely incorporated 3H-thymidine into the nucleus, and 3H-leucin into the cytoplasm, as well as in the rise of immunoreactive insulin concentration in the culture.
对取自10日龄豚鼠胰腺以及3至3.5月龄猪胚胎胰腺的胰岛单层培养物，在培养的第2至3天、第5至6天和第15至16天进行了研究。这些时间点分别对应培养物的强烈生长、稳定和生理性死亡阶段。通过3H-尿苷和3H-亮氨酸掺入的强度以及免疫反应性胰岛素分泌到培养基中的量，评估β细胞的胰岛素生物合成速率和分泌活性。通过选择性掺入3H-胸腺嘧啶核苷或3H-亮氨酸来检测增殖的和胰岛素合成的β细胞。在培养的强烈生长和死亡阶段，存活的β细胞分为胰岛素合成细胞和增殖细胞。在向稳定阶段过渡期间，β细胞的增殖水平急剧下降，而生物合成和胰岛素分泌速率增加。这表现为强烈将3H-胸腺嘧啶核苷掺入细胞核以及将3H-亮氨酸掺入细胞质的β细胞数量增加，以及培养物中免疫反应性胰岛素浓度升高。
