Tsoĭ T V, Sakanian V A, Lebedev A N, Alikhanian S I
Genetika. 1981;17(12):2100-4.
The promoter-containing fragments of Bacillus thuringiensis subsp. galleria 69-6 DNA have been cloned on the pGA24 vector in Escherichia coli cells. The recombinant plasmids make cells resistant to tetracycline in a wide range. The level of tetracycline-resistance does not depend on the length of a foreign insertion. New polypeptides are synthesized on the template of the recombinant plasmids in vitro. The data point out the presence in Bac. thuringiensis DNA of many genes which are able to express in E. coli cells.
苏云金芽孢杆菌亚种蜡螟变种69 - 6 DNA含启动子片段已在大肠杆菌细胞中克隆到pGA24载体上。重组质粒使细胞在很宽范围内对四环素具有抗性。四环素抗性水平不依赖于外源插入片段的长度。新的多肽在重组质粒模板上进行体外合成。这些数据表明苏云金芽孢杆菌DNA中存在许多能够在大肠杆菌细胞中表达的基因。
