Consolazione A, Milstein C, Wright B, Cuello A C
J Histochem Cytochem. 1981 Dec;29(12):1425-30. doi: 10.1177/29.12.7033368.
The development of the monoclonal antibody YC5/45 HLK (YC5/HLK) against a 5HT-bovine seroalbumin immunogen and its application for immunocytochemistry is described. The YC5/HLK antibody is the product of a rat x rat hybrid myeloma, producing a heavy chain and two light chains. In hemagglutination tests, the antibody cross-reacts to entirety with dopamine, serotonin, and tryptamine at high concentrations. The serotonin-albumin conjugate is 20,000 times more effective in displacing the binding antibody, while albumin itself goes unrecognized by the antibody. In fixed preparations of brain tissue, immunofluorescence is observed only in neurons known to contain serotonin, while no reaction is observed in dopamine-rich neurons. All immunofluorescence is extinguished by the use of agents that inhibit the biosynthesis of 5HT, but not of the catecholamines.
本文描述了针对5-羟色胺-牛血清白蛋白免疫原的单克隆抗体YC5/45 HLK（YC5/HLK）的研制及其在免疫细胞化学中的应用。YC5/HLK抗体是大鼠×大鼠杂交骨髓瘤的产物，产生一条重链和两条轻链。在血凝试验中，该抗体在高浓度下与多巴胺、5-羟色胺和色胺完全交叉反应。5-羟色胺-白蛋白偶联物在置换结合抗体方面的效力比白蛋白本身高20000倍，而白蛋白本身不被该抗体识别。在固定的脑组织标本中，仅在已知含有5-羟色胺的神经元中观察到免疫荧光，而在富含多巴胺的神经元中未观察到反应。使用抑制5-羟色胺生物合成但不抑制儿茶酚胺生物合成的试剂可消除所有免疫荧光。
