Spitzer G, Verma D S, Beran M, Zander A R, McCredie K B, Dicke K A
J Natl Cancer Inst. 1982 Jan;68(1):35-42.
The effects of coincubation of normal nonadherent bone marrow cells on adherent monolayers created from human peripheral blood mononuclear cells or marrow cells were investigated. Nonadherent marrow cells were coincubated for 4 hours with peripheral blood adherent cells at ratios of adherent cells to marrow cells of 2:1 to 5:1. This coincubation suppressed subsequent neutrophilic agar colony growth but not eosinophilic growth. Further studies suggested that this suppression was a cell-cell-mediated process and not secondary to soluble factors. However, coincubation on marrow adherent cells caused increased neutrophilic colony recovery. The possible in vivo relevance is discussed.
研究了正常非贴壁骨髓细胞与由人外周血单个核细胞或骨髓细胞形成的贴壁单层细胞共同孵育的效果。非贴壁骨髓细胞与外周血贴壁细胞以贴壁细胞与骨髓细胞2:1至5:1的比例共同孵育4小时。这种共同孵育抑制了随后嗜中性粒细胞琼脂集落的生长，但不影响嗜酸性粒细胞集落的生长。进一步的研究表明，这种抑制是细胞间介导的过程，并非由可溶性因子介导。然而，与骨髓贴壁细胞共同孵育会使嗜中性粒细胞集落回收率增加。文中讨论了其可能的体内相关性。
