Belitsina N V, Tnalina G Zh
Mol Biol (Mosk). 1981 Nov-Dec;15(6):1234-44.
Translation by RNA 4 from plant brome mosaic virus coding for the virus coat protein in an E. coli cell-free system with pure factors of translation has been studied. It has been shown that the initiation of translation by this mRNA depends completely on the three E. coli initiation factors. Optimal ionic conditions for the formation of the initiatory 70S times fMet-tRNA times RNA 4 complex have been found. It has been shown that this complex is stable in conditions of zonal centrifugation. On the basis of reaction with puromycin it has been determined that the initiatory fMet-tRNA in this complex occupies the donor-tRNA-binding site of the ribosome. By the competence of the initiatory ribosomal complex for binding with Ser-tRNA (serine is the N-terminal amino acid in the virus coat protein) it can be concluded that the ribosomal and the E. coli initiation factors recognize the initiatory codon of the RNA r from brome mosaic virus. Peptide synthesis induced by RNA 4 has been obtained on E. coli ribosomes with five pure factors of translation: IF-1, IF-2A, IF-3, EF-Tu or (Tu--Ts) and EF-G. The dependence of elongation on the Mg2+ concentration in the medium at RNA 4 translation has been determined.
在含有纯翻译因子的大肠杆菌无细胞系统中，对编码植物雀麦花叶病毒外壳蛋白的RNA 4的翻译进行了研究。结果表明，该mRNA的翻译起始完全依赖于三种大肠杆菌起始因子。已发现形成起始70S·fMet-tRNA·RNA 4复合物的最佳离子条件。结果表明，该复合物在区带离心条件下是稳定的。基于与嘌呤霉素的反应，已确定该复合物中的起始fMet-tRNA占据核糖体的供体tRNA结合位点。根据起始核糖体复合物与Ser-tRNA（丝氨酸是病毒外壳蛋白的N端氨基酸）结合的能力，可以得出核糖体和大肠杆菌起始因子识别来自雀麦花叶病毒的RNA 4的起始密码子的结论。利用五种纯翻译因子：IF-1、IF-2A、IF-3、EF-Tu或（Tu-Ts）和EF-G，在大肠杆菌核糖体上获得了由RNA 4诱导的肽合成。已确定在RNA 4翻译时延伸对培养基中Mg2+浓度的依赖性。
