Davies J W
Ann Microbiol (Paris). 1976 Jan;127A(1):131-42.
The multipartite nature of the brome mosaic virus genome is described. Particular attention is given to the translation of the RNAs in cell-free extracts, from dry wheat seed embryos and commercial wheat germ. Such studies have demonstrated that the smallest RNA is a monocistronic message for coat protein. This cistron is also carried on the intermediate sized RNA, which also codes for a larger protein, of unknown function. Translation of the larger RNAs 1 and 2 is compared, incell-free extracts of wheat embryo and wheat germ. There is evidence that these RNAs may also be monocistronic messages for large polypeptides (molecular weight 100,000), indicating that there are few non-translatable regions on these RNAs. Some recent nucleotide sequence studies are described, including the ribosome binding site of the coat protein cistron, and the 3' termini of all four RNAs, which appear to be identical, at least over a region of 150 nucleotides.
描述了雀麦花叶病毒基因组的多部分性质。特别关注了来自干燥小麦种子胚和商业小麦胚芽的无细胞提取物中RNA的翻译。此类研究表明，最小的RNA是衣壳蛋白的单顺反子信息。这个顺反子也存在于中等大小的RNA上，该RNA还编码一种功能未知的更大蛋白质。比较了小麦胚和小麦胚芽的无细胞提取物中较大的RNA 1和RNA 2的翻译情况。有证据表明，这些RNA可能也是大的多肽（分子量100,000）的单顺反子信息，这表明这些RNA上几乎没有不可翻译的区域。描述了一些最近的核苷酸序列研究，包括衣壳蛋白顺反子的核糖体结合位点，以及所有四种RNA的3'末端，这些末端至少在150个核苷酸的区域内似乎是相同的。
