Overton J, Meyer R, Chernoff E A
Scan Electron Microsc. 1981(Pt 2):297-305.
An epithelial sheet maintains its normal morphology by an appropriate balance between cell to cell and cell to substrate contacts which must be adjusted as proliferation occurs. We review our studies which attempts to analyze some of these problems by recombining cell and matrix tissue components, or by analyzing developmental changes in intact tissue. Epithelial and mesenchymal cells placed on tissue matrices which have been denuded of their normal tissue components behave distinctively, suggesting that this procedure could reveal inherent differences. When chick epiblast is grown on the outer surface of the vitelline membrane, the mode of expansion changes from that seen in the normal site, the vitelline membrane inner surface. Dispersed skin and corneal cells studied during subsequent aggregation show that one cell can influence desmosome formation in another. Finally, recent studies on developing chick liver indicate a correlation between the percent of lateral cell surface in the junctional domain and the proliferative rate of these cells.
上皮细胞层通过细胞间和细胞与基质间接触的适当平衡来维持其正常形态，随着细胞增殖，这种平衡必须进行调整。我们回顾了我们的研究，这些研究试图通过重组细胞和基质组织成分，或通过分析完整组织中的发育变化来分析其中的一些问题。置于已去除正常组织成分的组织基质上的上皮细胞和间充质细胞表现出明显不同的行为，这表明该程序可能揭示内在差异。当鸡胚外胚层在卵黄膜外表面生长时，其扩张模式与在正常部位即卵黄膜内表面所见的不同。对随后聚集过程中分散的皮肤和角膜细胞的研究表明，一个细胞可以影响另一个细胞中桥粒的形成。最后，对发育中的鸡肝脏的最新研究表明，连接域中细胞侧面表面积的百分比与这些细胞的增殖率之间存在相关性。
