Carstairs J R, Edwards D C, Pearce F L, Vernon C A, Walter S J
Eur J Biochem. 1977 Jul 15;77(2):311-7. doi: 10.1111/j.1432-1033.1977.tb11669.x.
Experiments are described that confirm the presence of gamma-globulin in standard preparations of mouse nerve growth factor (7-S complex and beta subunit) and show that antibodies to this protein are present in horse antisera to the growth factor. These antibodies may be partially removed by adsorption with soluble antigen and totally removed by affinity chromatography on columns of insolubilised antigen. These procedures do not affect the potency of the antiserum in vitro or in vivo. Contaminating globulin may be removed from samples of the isolated beta subunit by gel filtration to give an immunochemically pure preparation which will permit more meaningful studies on nerve growth factor and its antiserum.
所描述的实验证实了在小鼠神经生长因子的标准制剂（7-S复合物和β亚基）中存在γ-球蛋白，并表明在针对该生长因子的马抗血清中存在针对这种蛋白质的抗体。这些抗体可通过与可溶性抗原吸附而部分去除，并通过在不溶性抗原柱上进行亲和层析而完全去除。这些程序不会影响抗血清在体外或体内的效力。通过凝胶过滤可以从分离的β亚基样品中去除污染的球蛋白，从而得到免疫化学纯的制剂，这将有助于对神经生长因子及其抗血清进行更有意义的研究。
