Fukase M, Birge S J, Rifas L, Avioli L V, Chase L R
Endocrinology. 1982 Mar;110(3):1073-5. doi: 10.1210/endo-110-3-1073.
Primary monolayer cultures of cells from normal mouse kidney were initiated and maintained in serum-free medium supplemented with insulin, transferrin and prostaglandin E1. Renal epithelial cells grow to confluence without detectable growth of fibroblasts. These cells contain an active 25(OH)D3 1-hydroxylase with half-maximal formation of 1,25(OH)2D3 achieved at a substrate concentration of 13.3 nM. Activity of this enzyme is increased by low calcium medium or parathyroid hormone and decreased by high calcium medium, high phosphate medium or 1,25(OH)2D3.
从正常小鼠肾脏获取的细胞进行原代单层培养，并在添加胰岛素、转铁蛋白和前列腺素E1的无血清培养基中维持培养。肾上皮细胞生长至汇合状态，未检测到成纤维细胞生长。这些细胞含有一种活性25(OH)D3 1-羟化酶，在底物浓度为13.3 nM时可达到1,25(OH)2D3形成的半最大值。该酶的活性在低钙培养基或甲状旁腺激素作用下增加，而在高钙培养基、高磷培养基或1,25(OH)2D3作用下降低。
