Dolais-Kitabgi J, Faure T, Le Cam A
Mol Cell Endocrinol. 1981 Dec;24(3):315-23. doi: 10.1016/0303-7207(81)90006-x.
The effect of adrenalectomy on basal and hormone-stimulated amino-acid transport in liver was investigated by measuring the uptake of alpha-aminoisobutyric acid in freshly isolated rat hepatocytes. Adrenalectomy resulted in a 50% decrease in the transport capacity of hepatocytes; this change was accounted for by a diminution in the Vmax of a low affinity component of transport and specifically affected the A-transport system. Cortisone therapy fully restored the capacity of hepatocytes to transport amino acids. Sensitivity and responsiveness of hepatocytes from adrenalectomized animals to insulin, glucagon and dexamethasone, tested for the capacity of these hormones to stimulate AIB transport in vitro, were essentially identical with that of hepatocytes from control rats. The results support the concept of a positive (stimulatory) role of glucocorticoids in vivo, in the regulation of amino acid transport in the liver.
通过测量新鲜分离的大鼠肝细胞对α-氨基异丁酸的摄取，研究了肾上腺切除术对肝脏基础和激素刺激的氨基酸转运的影响。肾上腺切除术导致肝细胞转运能力下降50%；这种变化是由转运低亲和力成分的Vmax降低引起的，并且特别影响A转运系统。可的松治疗完全恢复了肝细胞转运氨基酸的能力。对肾上腺切除动物的肝细胞对胰岛素、胰高血糖素和地塞米松的敏感性和反应性进行了测试，检测这些激素在体外刺激AIB转运的能力，结果与对照大鼠肝细胞基本相同。这些结果支持了糖皮质激素在体内对肝脏氨基酸转运调节中起积极（刺激）作用的观点。
