Kay E D, Cheung C C, Jester J V, Nimni M E, Smith R E
Invest Ophthalmol Vis Sci. 1982 Feb;22(2):200-12.
The primary cultures obtained from the experimentally induced retrocorneal fibrous membrane synthesized and secreted into the medium mainly type I procollagen. This collagen was characterized after limited pepsin treatment and identified as type I collagen by the following criteria: (1) it contained two alpha 1 chains and one alpha 2 chain, (2) its sedimentation behavior was identical to that of type I collagen from skin, and (3) its peptide map after limited proteolysis with Staphylococcus aureus V8 protease was identical to that of type I collagen. The medium contained procollagen I, which was converted into alpha size chains by limited pepsin treatment, whereas the cellular fraction contained type I collagen already processed to its end product. Type III collagen and basement membrane collagen were present as minor components in this system Fibronectin, one of the major glycoproteins in extracellular matrices, was also synthesized and secreted into the medium. In contrast, normal corneal endothelial cells produce mainly basement membrane collagen.
从实验诱导的角膜后纤维膜获得的原代培养物主要合成并分泌I型前胶原到培养基中。经有限的胃蛋白酶处理后对这种胶原进行了表征，并通过以下标准鉴定为I型胶原：（1）它含有两条α1链和一条α2链，（2）其沉降行为与来自皮肤的I型胶原相同，（3）用金黄色葡萄球菌V8蛋白酶进行有限蛋白水解后的肽图与I型胶原相同。培养基中含有I型前胶原，经有限的胃蛋白酶处理可转化为α大小的链，而细胞部分含有已加工成终产物的I型胶原。III型胶原和基底膜胶原在该系统中作为次要成分存在。细胞外基质中的主要糖蛋白之一纤连蛋白也被合成并分泌到培养基中。相比之下，正常角膜内皮细胞主要产生基底膜胶原。
