Insel R A, Anderson P W
J Immunol. 1982 Mar;128(3):1267-70.
Escherichia coli K100 is known to induce antibodies cross-reactive with the capsular polysaccharide (CP) of Haemophilus influenzae b (Hib); the cross-immunogenicity is found consistently in a number of mammalian species including man. We have studied the reciprocal cross-immunogenicity of Hib or its purified CP in man. Of 25 adults and 13 children responding to systemic immunization with Hib CP, only four and four, respectively, made K100 CP cross-reactive antibody, determined by radioantigen-binding inhibition. The detection of preimmunization cross-reactive antibody was not statistically predictive of induction of postimmunization cross-reactive antibody. Cross-reactive antibody was detected in only one of 11 children making an anticapsular response to Hib systemic infection and two of four children responding to asymptomatic nasopharyngeal colonization. In contrast, cross-reactivity was detected in the naturally occurring anti-Hib-CP antibody of 27 of 42 children. Thus, humans responding to Hib or its CP often do not make antibody to the K100-cross-reactive determinant(s), implying that the cross-reactive natural antibodies frequently seen in children must have a stimulus other than Hib.
已知大肠杆菌K100可诱导产生与b型流感嗜血杆菌（Hib）的荚膜多糖（CP）发生交叉反应的抗体；在包括人类在内的许多哺乳动物物种中均一致发现了这种交叉免疫原性。我们研究了Hib或其纯化的CP在人类中的相互交叉免疫原性。在用Hib CP进行全身免疫的25名成年人和13名儿童中，分别只有4名成年人和4名儿童产生了K100 CP交叉反应性抗体，这是通过放射抗原结合抑制法测定的。免疫前交叉反应性抗体的检测在统计学上并不能预测免疫后交叉反应性抗体的诱导情况。在11名对Hib全身感染产生抗荚膜反应的儿童中，只有1名检测到交叉反应性抗体，在4名对无症状鼻咽部定植有反应的儿童中，只有2名检测到交叉反应性抗体。相比之下，在42名儿童中，有27名儿童的天然抗Hib-CP抗体中检测到了交叉反应性。因此，对Hib或其CP有反应的人类通常不会产生针对K100交叉反应决定簇的抗体，这意味着在儿童中经常见到的交叉反应性天然抗体必定有除Hib之外的刺激因素。
