Frangakis M V, Koopman W J, Kiyono H, Michalek S M, McGhee J R
J Immunol Methods. 1982;48(1):33-44. doi: 10.1016/0022-1759(82)90207-1.
Enzyme (Dispase) dissociation of murine Peyer's patches (PP) 3 yields a cell population rich in T and B lymphocytes and containing 5-9% macrophages (MO) as defined by morphology and esterase staining. Cell yields by this method are 3-4 times greater than with conventional mechanical methods and viability of cells always exceeds 95%. The virtual absence of IgA plasma cells (less than 1%) indicates lack of contamination of the cell preparations with intestinal mucosal cells. Enzymatically dissociated PP cells proliferate in response to both T and B cell mitogens. Differences in patterns of mitogen responsiveness of sequentially obtained PP cell fractions are observed suggesting considerable functional heterogeneity of PP cells. Patterns of IgM and IgA synthesis by PP cells in response to the B cell polyclonal activator LPS differ considerably from spleen cells. The development of methodology for preparation of PP cells containing functionally intact T and B cells and enriched for MO should permit analysis of the cellular basis for functional specialization of PP.
用酶（Dispase）解离小鼠派尔集合淋巴结（PP）3可产生富含T和B淋巴细胞的细胞群体，根据形态学和酯酶染色确定其中含有5 - 9%的巨噬细胞（MO）。通过这种方法获得的细胞产量比传统机械方法高3 - 4倍，细胞活力始终超过95%。几乎不存在IgA浆细胞（少于1%）表明细胞制剂未被肠黏膜细胞污染。酶解的PP细胞对T和B细胞有丝分裂原均有增殖反应。观察到连续获得的PP细胞组分对有丝分裂原反应模式的差异，提示PP细胞存在相当大的功能异质性。PP细胞对B细胞多克隆激活剂LPS的IgM和IgA合成模式与脾细胞有很大不同。制备含有功能完整的T和B细胞且富含MO的PP细胞的方法的发展，应有助于分析PP功能特化的细胞基础。
